Journal: Scientific reports
Article Title: Exercise promotes peripheral glycolysis in skeletal muscle through miR-204 induction via the HIF-1α pathway.
doi: 10.1038/s41598-025-85174-0
Figure Lengend Snippet: Fig. 3. Hepatic lipid decreased by chronic exercise is not related with miR-204. (A) Serum TG (triglycerides; mg/dl) and FFA (free fatty acids; ng/ml) of chronic exercise Con (control) and TM (Treadmill) groups. (B) Ratio of liver weight per body weight (%) of chronic exercise Con and TM groups. (C) H&E (hematoxylin and eosin) and Oil-Red-O staining of livers of chronic exercise Con and TM groups. Liver TG levels (mg/g tissue) of chronic exercise Con and TM groups. (D) Western blot analysis and quantification of lipogenesis (SREBP-1, pACC, and ACC) and fatty acid transporter (CD36) enzymes in livers of chronic exercise Con and TM groups. HSP90 was used for an internal control. (E) miR-204 expression levels in livers of chronic exercise Con and TM groups. Rplp0 was used for an internal control. (F) miR-204 expression levels of control (normal, 12), NASH (9), and cholestatic (13) patients from GEO dataset (GSE49012).
Article Snippet: Primary antibodies used: HK2, PKM2, PDH, LDH (#8337, Cell Signaling Technology, Danvers, United States), HSP90 (#4874, Cell Signaling Technology), HIF1α (A7684, Abclonal), SREBP-1 (sc-13551, Santa Cruz Biotechnology, CA, United States), CD36 (A5792, Abclonal, Woburn, Unisted States), pACC (#3661, Cell Signaling Technology), ACC (#3676, Cell Signaling Technology).
Techniques: Control, Staining, Western Blot, Expressing